Estrone ELISA: A Quantitative Measurement Tool for Estrogen Hormone in Reproductive Health Research

The Estrone ELISA (enzyme-linked immunosorbent assay) is a diagnostic tool developed by Demeditec Diagnostics GmbH, a reputable manufacturer based in Germany. This ELISA method offers a reliable and accurate quantitative measurement of Estrone, a naturally occurring estrogen hormone, in serum and plasma samples.

Understanding the Importance of Estrone

Estrone plays a crucial role in various physiological processes, particularly within the reproductive system. It is involved in regulating hormonal balance and supporting reproductive health. Researchers and clinicians studying reproductive health and related disorders can benefit greatly from utilizing this valuable tool.

Efficient 96-Well Plate Format

The Estrone ELISA utilizes a convenient 96-well plate format, allowing for efficient processing of multiple samples simultaneously. With an incubation time of 1 hour and 15 minutes at room temperature, this method ensures timely results without compromising accuracy.

Precise Quantification and Sensitivity

The standard range for the assay spans from 15 to 2,400 pg/ml, providing a comprehensive scope for measuring Estrone levels in samples.
The sensitivity of the assay is an impressive 8.1 pg/ml, enabling precise detection even at low concentrations.

Simplified Sample Handling

A final sample volume of only 25 µl is required, minimizing the amount of sample needed while maintaining accurate measurements.
The accepted sample types include serum and plasma (EDTA), which are commonly used in research and clinical settings.
No sample pretreatment is necessary, streamlining the workflow and saving valuable time.

Reliable Estrone Detection with Internal Controls

The detection of Estrone is achieved using TMB (3,3',5,5'-tetramethylbenzidine) as the substrate. The absorbance is measured at 450 nm, ensuring reliable and consistent results. Additionally, this ELISA includes internal controls to validate the accuracy and reliability of each assay run.

Compliance with European Regulatory Standards

The Estrone ELISA by Demeditec Diagnostics GmbH is CE marked, indicating its compliance with European regulatory standards. This certification ensures that the product meets strict quality requirements and can be confidently used in research and clinical applications.

In summary, the Estrone ELISA provides researchers and clinicians with a powerful tool for quantitative measurement of Estrone in serum and plasma samples. Its efficient 96-well plate format, precise quantification, simplified sample handling, reliable detection, and compliance with European regulatory standards make it an invaluable asset in reproductive health research and related disorders.